Confirmation and Keplerian motion of the gap-carving protoplanet HD 169142 b

We present the re-detection of a compact source in the face-on protoplanetary disc surrounding HD 169142, using VLT/SPHERE data in YJH bands. The source is found at a separation of 0.''319 ($\sim$37 au) from the star. Three lines of evidence argue in favour of the signal tracing a protoplanet: (i) it is found in the annular gap separating the two bright rings of the disc, as predicted by theory; (ii) it is moving at the expected Keplerian velocity for an object at $\sim$37 au in the 2015, 2017 and 2019 datasets; (iii) we also detect a spiral-shaped signal whose morphology is consistent with the expected outer spiral wake triggered by a planet in the gap, based on dedicated hydrodynamical simulations of the system. The YJH colours we extracted for the object are consistent with tracing scattered starlight, suggesting that the protoplanet is enshrouded in a significant amount of dust, as expected for a circumplanetary disc or envelope surrounding a gap-clearing Jovian-mass protoplanet.Comment: Accepted for publication in MNRAS Letters. 5 pages, 5 figure

Exoplanet imaging data challenge, phase II: characterization of exoplanet signals in high-contract images

peer reviewedToday, there exists a wide variety of algorithms dedicated to high-contrast imaging, especially for the detection and characterisation of exoplanet signals. These algorithms are tailored to address the very high contrast between the exoplanet signal(s), which can be more than two orders of magnitude fainter than the bright starlight residuals in coronagraphic images. The starlight residuals are inhomogeneously distributed and follow various timescales that depend on the observing conditions and on the target star brightness. Disentangling the exoplanet signals within the starlight residuals is therefore challenging, and new post-processing algorithms are striving to achieve more accurate astrophysical results. The Exoplanet Imaging Data Challenge is a community-wide effort to develop, compare and evaluate algorithms using a set of benchmark high-contrast imaging datasets. After a first phase ran in 2020 and focused on the detection capabilities of existing algorithms, the focus of this ongoing second phase is to compare the characterisation capabilities of state-of-the-art techniques. The characterisation of planetary companions is two-fold: the astrometry (estimated position with respect to the host star) and spectrophotometry (estimated contrast with respect to the host star, as a function of wavelength). The goal of this second phase is to offer a platform for the community to benchmark techniques in a fair, homogeneous and robust way, and to foster collaborations

Impact des ApoExos dans le bris de la tolérance aux antigènes vasculaires et au déclenchement d’une réponse auto-immune systémique

Les exosomes apoptotiques (ApoExo) sont des vésicules extracellulaires (EVs) dérivées de lésions vasculaires et libérées par des cellules endothéliales (ECs) apoptotiques dont la taille, les protéines, le profil en ARN et l'activité enzymatique sont différents de ceux des corps apoptotiques classiques. Notre groupe a montré que les ApoExos accéléreraient le rejet vasculaire en association avec les anti-LG3 circulants, des auto-anticorps (auto-Ac) dirigés contre le LG3, le fragment 5' du perlécan. Nous avons également démontré le rôle de biomarqueur et le rôle effecteur des anti-LG3 dans les lésions vasculaires rénales, à la fois dans les reins natifs et transplantés. La néphrite lupique (NL) est une manifestation fréquente et grave du lupus érythémateux disséminé (LED). Il n'existe pas de biomarqueurs du dysfonctionnement rénal progressif dans la NL. Nous émettons l'hypothèse que les ApoExos stimulent des cellules B spécifiques qui existent dans le répertoire immunitaire normal et que les conditions pro-inflammatoires prévalant chez les patients atteints de LED, telles que l'activation accrue des récepteurs Toll-like (TLRs), amplifient cette réponse, conduisant à la production d'anti-LG3, un auto-Ac important dans l'établissement de la NL. Des cellules B productrices d’anti-LG3 ont été trouvées dans la cavité péritonéale de souris saines et ont produit des anti-LG3 suite à une stimulation in vitro avec des agonistes des TLR1/2, TLR4, TLR7 et TLR9. Il est intéressant de noter que ces cellules sont absentes de la cavité péritonéale de souris saines ayant reçu une injection d'ApoExos. En explorant l'importance fonctionnelle des TLRs dans le déclenchement d'une réponse auto-immune dans un modèle murin lupique, nous montrons que les agonistes de TLRs connus pour contribuer à la pathogenèse du LED (TLR2, 4, 7 et 9) déclenchent une production significativement plus élevée d'IgM anti-LG3, alors que la stimulation des TLRs qui ne sont pas associés à la pathogenèse du LED (TLR3 et 5) ne le fait pas. L’injection d'ApoExo a également déclenché l'axe auto-immun IL-23/IL-17 (mesuré par ELISA et essai cytokinique), augmenté les cellules B de centres germinatifs spléniques (mesuré par cytométrie de flux), augmenté les taux circulants d’IgG totaux, d’anti-LG3 et d’auto-Ac classiques du LED (mesuré par micropuce et ELISA) par rapport à l’injection de véhicule. Des niveaux élevés d'IgG anti-LG3 circulants sont observés chez les souris prédisposées au LED par rapport aux souris saines (mesurés par ELISA), ainsi qu'une proportion accrue de cellules B1 spléniques et de cavité péritonéale (mesurés par cytométrie de flux) augmentant avec l’établissement de la maladie. Ces observations suggèrent un rôle spécifique des ApoExos dans la modulation de la production d'auto-Ac qui, à son tour, déclenche l'involution microvasculaire importante dans les maladies auto-immunes et le rejet de greffe. Ces observations suggèrent également que les cellules B spécifiques de LG3 peuvent être modulées dans des conditions pro-inflammatoires telles que celles qui prévalent chez les patients atteints de LED, conduisant à la production d'auto-Ac. Une meilleure compréhension de l'impact de ces mécanismes permettra d'améliorer l'identification, la prédiction et la prise en charge de la NL.Apoptotic exosomes (ApoExo) are vascular injury derived extracellular vesicles (EVs) released by apoptotic endothelial cells (ECs) with distinct size, protein, RNA profile and enzymatic activity from classical apoptotic bodies. Our group showed that ApoExo accelerated vascular rejection in association with circulating anti-LG3, autoantibodies (autoAb) against LG3, the 5’ fragment of the perlecan. We have also unravelled biomarkers and effector roles of anti-LG3 in kidney vascular damage in both native and transplanted kidneys. Lupus Nephritis (LN) is a common and serious manifestation of systemic lupus erythematosus (SLE). Biomarkers of progressive renal dysfunction in LN, are lacking. We hypothesize that ApoExo stimulate specific B cells that exist in the normal immune repertoire and that the pro-inflammatory conditions prevalent in SLE patients, such as increased Toll-like Receptors (TLRs) activation, amplify this response, leading to anti-LG3 production, autoAb of importance in LN development. B cells producing anti-LG3 were found in the peritoneal cavity of healthy mice and produced anti-LG3 AutoAb when stimulated in vitro with TLR 1/2, 4, 7 and 9 agonists. Interestingly, these cells disappeared from the peritoneal cavity of healthy mice infused with ApoExo. ApoExo infusion also triggered circulating IL-23/IL-17 autoimmune axis (measured by cytokines assay), increased splenic germinal centre B cells (measured by flow cytometry), increased total circulating IgG, anti-LG3 and classical autoAb (measured by microarray and ELISA) compared to vehicle infusion. Elevated circulating anti-LG3 IgG levels are found in SLE prone mice compared to healthy ones (measured by ELISA) as well as an increased proportion of splenic and peritoneal cavity B1 cells (measured by flow cytometry). Exploring the functional importance of TLRs in triggering such a response, we show that while TLR agonists known to contribute to SLE pathogenesis (TLR2, 4, 7 and 9) triggered significantly higher IgM anti-LG3 production, stimulation of TLR that are not associated with SLE pathogenesis (TLR3 and 5) did not. These observations suggest a specific role for ApoExo in modulating the production of autoAb which, in turn, trigger microvascular involution of importance in autoimmune diseases and transplant rejection. These observations also suggest that LG3-specific B cells may be modulated under pro-inflammatory conditions such as those prevalent in lupus patients, leading to production of autoAb. A better understanding of the impact of these mechanisms will lead to improved identification, prediction, and management of LN

Étude structurale et dynamique de la myoglobine (insertion d'hèmes artificiels)

De part leurs diverses fonctions, les hémoprotéines ont été le sujet de nombreuses recherches centrées principalement sur la compréhension de la relation structure-fonction. Les résultats obtenus permettent, aujourd hui, de construire des protéines artificielles avec la structure et/ou la fonction souhaitées. Cependant, des phénomènes structuraux restent aujourd hui à élucider, notamment la dynamique de l hème. Dans le but de répondre à cette question, nous avons envisagé des myoglobines artificielles avec des hèmes de petite taille, dérivés de la porphine. Dans un premier temps, des b-alkylporphyrines de fer et méso-monophénylporphyrines de fer ont été synthétisées et caractérisées. La myoglobine est ensuite reconstituée avec deux de ces complexes: la 3,7-diéthyl-2,8-diméthylporphyrine de fer et la 3-éthyl-2-méthylporphyrine de fer. Les études RMN démontrent que la fixation de l hème est liée à l encombrement des substituants et sa perte de symétrie.RENNES1-BU Sciences Philo (352382102) / SudocSudocFranceF

Analysis of the arm-like structure in the outer disk of PDS 70. Spiral density wave or vortex?

peer reviewedContext. Observing dynamical interactions between planets and disks is key to understanding their formation and evolution. Two protoplanets have recently been discovered within the PDS 70 protoplanetary disk, along with an arm-like structure toward the northwest of the star. Aims: Our aim is to constrain the morphology and origin of this arm-like structure, and to assess whether it could trace a spiral density wave caused by the dynamical interaction between the planet PDS 70c and the disk. Methods: We analyzed polarized and angular differential imaging (PDI and ADI) data taken with VLT/SPHERE, spanning six years of observations. The PDI data sets were reduced using the irdap polarimetric data reduction pipeline, while the ADI data sets were processed using mustard, a novel algorithm based on an inverse problem approach to tackle the geometrical biases spoiling the images previously used for the analysis of this disk. Results: We confirm the presence of the arm-like structure in all PDI and ADI data sets, and extract its trace by identifying local radial maxima in azimuthal slices of the disk in each data set. We do not observe a southeast symmetric arm with respect to the disk minor axis, which seems to disfavor the previous hypothesis that the arm is the footprint of a double-ring structure. If the structure traces a spiral density wave following the motion of PDS 70c, we would expect 11°.28−0°.86+2°.20 rotation for the spiral in six years. However, we do not measure any significant movement of the structure. Conclusions: If the arm-like structure is a planet-driven spiral arm, the observed lack of rotation would suggest that the assumption of rigid-body rotation may be inappropriate for spirals induced by planets. We suggest that the arm-like structure may instead trace a vortex appearing as a one-armed spiral in scattered light due to projection effects. The vortex hypothesis accounts for both the lack of observed rotation and the presence of a nearby sub-millimeter continuum asymmetry detected with ALMA. Additional follow-up observations and dedicated hydrodynamical simulations could confirm this hypothesis

Proton NMR Study of low-Spin meso-unsubstituted Beta-substituted alkyl Iron Porphyrins: remarkable influence of peripheral substitution on spin density

International audienc

Dynamics of heme in hemoproteins: proton NMR study of myoglobin reconstituted with iron 3-ethyl-2-methylporphyrin.

International audienceThe asymmetric 3-ethyl-2-methylporphyrin iron complex was synthetized and inserted into apomyoglobin. UV-visible spectroscopic studies demonstrated the capacity of iron to coordinate different exogenous axial ligands in ferrous and ferric forms. The position of synthetic heme into the hydrophobic pocket of the reconstituted myoglobin was investigated by ((1))H NMR spectroscopy. In absence of exogenous ligand, signals of the synthetic prosthetic group were not detected, suggesting a rotational disorder of the synthetic porphyrin into the heme pocket. This direct interconversion behavior is favored since site-specific interactions between the poorly substituted heme and protein in the chiral hydrophobic cavity were weak. Complexion of cyanide to the iron allowed to quench partially the heme reorientation and two interconvertible forms, around the meso-Cα-Cγ axis, were detected in solution

Building the EnCOrE dictionary collaboratively: strategy and practice

We are building an encyclopaedia of organic chemistry (EnCOrE) in English and making it freely available on the internet. We expect the tools to be used by novice as well as advanced researchers from university and industry. They will allow cross-searches based on chemical structures and equations, and on explanatory texts and an elaborated dictionary. EnCOrE will be created under a strong editorial organisation but also collaboratively between chemists. This will ensure that it is kept up to date and will favour a consensual approach. We will disclose our effort to develop, in this way, the EnCOrE dictionary and the protocols to favour collaboration